The standardized karyotype and idiogram of the pileated gibbon, Hylobates pileatus (Hylobatidae, Primates) by G-banding and high-resolution technique.

Supanuam, P., Tanomtong, A. and Khunsook, S. 2007. The standardized karyotype and idiogram of the pileated gibbon, Hylobates pileatus (Hylobatidae, Primates) by G-banding and high-resolution technique. Cytologia 72(2): 189-194. (ISI Impact Factor 2012 = 0.43) [PDF]

Summary

         Standardized karyotype and idiogram of the pileated gibbon (Hylobates pileatus) Nakhon Ratchasima Zoo, Thailand was studied. Blood sample were taken from 2 females and 2 males. After standard whole blood lymphocyte culture at 37°C for 72 h in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of pileated gibbon was 2n (diploid) 44. The type of autosomes were 30 metacentric,10 submetacentric and 2 acrocentric chromosomes, with X and Y chromosome being submetacentric and acrocentric chromosome, respectively. From the G-banding and high-resolution technique, the numbers of bands and locations in the pileated gibbon were 183 and 236 respectively, each chromosome pair could be clearly differentiated.

Key words: karyotype, idiogram, G-banding, high-resolution technique, pileated gibbon (Hylobates pileatus).