A Study on the Chromosomes of White-cheeked Gibbon, Nomascus leucogenys (Primate, Hylobatidae) by Conventional Staining Technique

Kaewsri, S., Tanomtong, A., Supanuam, P., Khunsook, S. and Sanoamuang, L. 2013. A Study on the Chromosomes of White-cheeked Gibbon, Nomascus leucogenys (Primate, Hylobatidae) by Conventional Staining Technique. KKU Science Journal 41(2) 394-402. [PDF] (TCI: Thai-Journal Citation Index Centre)

ABSTRACT

      Standardized karyotype and idiogram of white-cheeked gibbon (Nomascus leucogenys) at Nakhonratchasima Zoo, was study. Blood sample were taken from 1 female and 2 males gibbons. After standard whole blood lymphocyte culture at 37 C for 72 hr. in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining technique was applied to stain the chromosomes. The results show that diploid chromosome number is 2n = 52, and the fundamental number (NF) is 104 in both female and male. The autosomes consist of 22 large metacentric, 8 large submetacentric, 10 medium metacentric, 4 medium submetacentric and 6 small acrocentric chromosomes. The X-chromosome is the large acrocentric and the Y-chromosome is the smallest submetacentric chromosome. The karyotype formula for the white-cheeked gibbon is as follows:

          2n (52) = Lm22 Lsm8+Mm10+Msm4+Sa6 + sex-chromosomes

Keywords: Karyotype, Chromosome, Conventional staining, White-cheeked gibbon