A comparative chromosome analysis of Thai wild boar (Sus scrofa jubatus) and relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique

Tanomtong, A., Supanuam, P., Siripiyasing, P. and Bunjonrat, R. 2007. A comparative chromosome analysis of Thai wild boar (Sus scrofa jubatus) and relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique. Songklanakarin Journal of Science and Technology 29(1): 1-13. (SCOPUS Database) [PDF]

Abstract

           This research is the first comparative chromosome analysis report of Thai wild boar (Sus scrofa jubatus) and its relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique. Blood samples of the Thai wild boar were taken from two males and two females kept in Nakhon Ratchasima Zoo. After standard whole blood lymphocyte culture at 37 oC for 72 hr. in the presence of colchicine, the metaphase spreads were performed on microscopic slides and airdried.Conventional staining, G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of Thai wild boar was 2n (diploid) = 38, and the fundamental numbers (NF) were 62 in the male and female. The type of autosomes were 12 metacentric, 14 submetacentric, 4 acrocentric and 6 telocentric chromosomes, with X and Y chromosomes being metacentric chromosomes. We found that chromosomes 1, 5, 7, 8, 10, 11, 12, 13, 14, 16, 17, 18, X and Y had the same G-banding and high-resolution technique patterns as those of domestic pig chromosomes. Chromosomes 2, 3, 4, 6, 9 and 15 are similar to those of domestic pig chromosomes. These results show the evolutionary relationship between the Thai wild boar and the domestic pig.

Key words : chromosome, conventional staining, G-banding, high-resolution technique,

Thai wild boar (Sus scrofa jubatus), domestic pig (Sus scrofa domestica)