A Study on the Chromosomes of White-cheeked Gibbon, Nomascus leucogenys (Primate, Hylobatidae) by Conventional Staining Technique

Kaewsri, S., Tanomtong, A., Supanuam, P., Khunsook, S. and Sanoamuang, L. 2013. A Study on the Chromosomes of White-cheeked Gibbon, Nomascus leucogenys (Primate, Hylobatidae) by Conventional Staining Technique. KKU Science Journal 41(2) 394-402. [PDF] (TCI: Thai-Journal Citation Index Centre)

ABSTRACT

      Standardized karyotype and idiogram of white-cheeked gibbon (Nomascus leucogenys) at Nakhonratchasima Zoo, was study. Blood sample were taken from 1 female and 2 males gibbons. After standard whole blood lymphocyte culture at 37 C for 72 hr. in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining technique was applied to stain the chromosomes. The results show that diploid chromosome number is 2n = 52, and the fundamental number (NF) is 104 in both female and male. The autosomes consist of 22 large metacentric, 8 large submetacentric, 10 medium metacentric, 4 medium submetacentric and 6 small acrocentric chromosomes. The X-chromosome is the large acrocentric and the Y-chromosome is the smallest submetacentric chromosome. The karyotype formula for the white-cheeked gibbon is as follows:

          2n (52) = Lm22 Lsm8+Mm10+Msm4+Sa6 + sex-chromosomes

Keywords: Karyotype, Chromosome, Conventional staining, White-cheeked gibbon

First chromosome characterization in the large Indian civet, Viverra zibetha (Canivora, Viverridae) in Thailand by conventional staining and GTG-banded technique

 Tanomtong, A., Supanuam, P., Sangpakdee, W. and Aengwanich, W. 2008. First chromosome characterization in the large Indian civet, Viverra zibetha (Carnivora, Viverridae) in Thailand by Conventional staining and GTG-banded technique. Caryologia 61(3): 206-210. (ISI Impact Factor 2011 = 0.533) [PDF]

Abstract

            This research was the first cytogenetic study of large Indian civet (Viverra zibetha) in Thailand. Blood samples of the species were taken from two males and one female kept in Songkhla Zoo, Songkhla province, Thailand. After the standard whole blood lymphocyte culture in presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining and GTG-banded technique were applied to stain the chromosomes. The karyotype of large Indian civet show that diploid chromosome number was 2n=38, and the fundamental number (NF) were 72 in female and 71 in male. The autosomes consist of 4 large metacentric, 10 large submetacentric, 10 large acrocentric, 2 large telocentric, 2 medium metacentric, 2 medium acrocentric, 4 small acrocentric and 2 small telocentric chromosomes. The X chromosome was large submetacentric and the Y chromosome was the smallest telocentric chromosome. Idiogram represent banding pattern at the metaphase chromosome was 345 bands and found that chromosome pairs 13 was the satellite marker chromosome that have secondary constriction on long length arm. These results will be useful for future studies of breeding, conservation, chromosome evolution and basic genetics information in this species.

Key words: chromosome, karyotype, large Indian civet (Viverra zibetha).

The standardized karyotype and idiogram of the pileated gibbon, Hylobates pileatus (Hylobatidae, Primates) by G-banding and high-resolution technique.

Supanuam, P., Tanomtong, A. and Khunsook, S. 2007. The standardized karyotype and idiogram of the pileated gibbon, Hylobates pileatus (Hylobatidae, Primates) by G-banding and high-resolution technique. Cytologia 72(2): 189-194. (ISI Impact Factor 2012 = 0.43) [PDF]

Summary

         Standardized karyotype and idiogram of the pileated gibbon (Hylobates pileatus) Nakhon Ratchasima Zoo, Thailand was studied. Blood sample were taken from 2 females and 2 males. After standard whole blood lymphocyte culture at 37°C for 72 h in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of pileated gibbon was 2n (diploid) 44. The type of autosomes were 30 metacentric,10 submetacentric and 2 acrocentric chromosomes, with X and Y chromosome being submetacentric and acrocentric chromosome, respectively. From the G-banding and high-resolution technique, the numbers of bands and locations in the pileated gibbon were 183 and 236 respectively, each chromosome pair could be clearly differentiated.

Key words: karyotype, idiogram, G-banding, high-resolution technique, pileated gibbon (Hylobates pileatus).

A comparative chromosome analysis of Thai wild boar (Sus scrofa jubatus) and relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique

Tanomtong, A., Supanuam, P., Siripiyasing, P. and Bunjonrat, R. 2007. A comparative chromosome analysis of Thai wild boar (Sus scrofa jubatus) and relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique. Songklanakarin Journal of Science and Technology 29(1): 1-13. (SCOPUS Database) [PDF]

Abstract

           This research is the first comparative chromosome analysis report of Thai wild boar (Sus scrofa jubatus) and its relationship to domestic pig (S. s. domestica) by conventional staining, G-banding and high-resolution technique. Blood samples of the Thai wild boar were taken from two males and two females kept in Nakhon Ratchasima Zoo. After standard whole blood lymphocyte culture at 37 oC for 72 hr. in the presence of colchicine, the metaphase spreads were performed on microscopic slides and airdried.Conventional staining, G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of Thai wild boar was 2n (diploid) = 38, and the fundamental numbers (NF) were 62 in the male and female. The type of autosomes were 12 metacentric, 14 submetacentric, 4 acrocentric and 6 telocentric chromosomes, with X and Y chromosomes being metacentric chromosomes. We found that chromosomes 1, 5, 7, 8, 10, 11, 12, 13, 14, 16, 17, 18, X and Y had the same G-banding and high-resolution technique patterns as those of domestic pig chromosomes. Chromosomes 2, 3, 4, 6, 9 and 15 are similar to those of domestic pig chromosomes. These results show the evolutionary relationship between the Thai wild boar and the domestic pig.

Key words : chromosome, conventional staining, G-banding, high-resolution technique,

Thai wild boar (Sus scrofa jubatus), domestic pig (Sus scrofa domestica)