A study on karyotype of the pileated gibbon, Hylobates pileatus

(Primates, Hylobatidae), by conventional staining

Tanomtong, A., Supanuam, P. and Khunsook, S. 2008. A study on karyotype of the pileated gibbon,

Hylobates pileatus (Primates, Hylobatidae), by conventional staining. Songklanakarin Journal of Science and Technology 30(4): 421-426. [PDF] (SCOPUS Database)

Abstract

        Cytogenetics of the pileated gibbon (Hylobates pileatus) at Nakhon Ratchasima Zoo, Thailand, was studied. Blood samples were taken from two female and two male gibbons. After lymphocyte culture, the mitotic chromosome preparation was done by hypotonic-fixation-air-drying method and conventional Giemsa’s staining. The results show that diploid chromosome number was 44 (2n=2x=44), and the fundamental number (NF) were 88 chromosomes in both female and male. The autosomes consist of 12 large metacentric, 6 medium metacentric, 2 medium submetacentric, 2 medium acrocentric, 12 small metacentric and 8 small submetacentric chromosomes. In addition, the chromosome 15 showed clearly observable satellite chromosomes. The X chromosome was a medium submetacentric chromosome and the Y chromosome was a tiny acrocentric chromosome. The karyotype formula for the pileated gibbon is as follows:
           2n (44) = Lm12+Mm6+Msm2+Ma2+Sm12+Ssm8+sex-chromosomes

Key words: karyotype, pileated gibbon (Hylobates pileatus), conventional staining